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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been noticed in many tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial position in advertising and marketing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up noted but comprehensive nucleotide sequence data isn't nonetheless obtainable.
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Blocking peptides are peptides that bind specifically for the concentrate on antibody and block antibody binding. These peptide generally contains the epitope identified by the antibody.
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This tutorial outlines even more feasible optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
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This manual outlines additional feasible optimization of cellular and immunoassay parameters to make certain the very best success are...
Antibodies sure to the blocking peptide no more bind to your epitope within the goal protein. By comparing the staining within the blocked antibody compared to the antibody alone, you can see which staining is unique.
Target Information and facts Cyclin E belongs to the very conserved cyclin family members, whose users are characterised by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Various cyclins show unique expression and degradation designs which add towards the temporal coordination of each mitotic occasion. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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